Saturday, December 21, 2019

Aseptic Technique Lab Essay - 1386 Words

Aseptic Technique and Culturing Microbes Lab 3 Brittney Case August 4th 2013 Section: Mic 101 Microbiology Session 1 Abstract: The Purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what is required for them to grow properly. Hypothesis: This exercise will allow me to gain knowledge of how to properly use aseptic techniques, become familiar with basic requirements of microbial growth, cultured media and methods to control their growth. This will further my knowledge and skills in making observations about specimens. Methodology: Materials: -Small†¦show more content†¦B. List five microbial killing methods, how they work, and what they are used for. 1. Incineration: burns organisms and physically destroys them. This method is used for needles, inoculating wires, and glassware. 2. Boiling: at 100 degree C for 30 minutes kills almost all endospores. Very long or intermittent boiling is required to kill endospores and sterilize a solution. This method is most commonly used for purifying drinking water. 3. Dry Heat: (hot oven air) at 160 degree C for 2 hours or 170 degree C for 1 hour. This method is used for glassware, metal and objects that will not melt. 4. Irradiation: usually destroys or distorts nucleic acids. Ultraviolet light is generally used to sterilize the surfaces of objects, although x-rays and microwaves can be usefull. 5. Toxic chemicals and gas: such as formaldehyde, glutaraldehyde, and ethylene oxide can kill all forms of life in a specialized gas chamber. C. What is a pure culture? Why is it important to work with a pure culture? A pure culture is a culture containing a single species of organisms. It is important to work with a pure culture because it ensures that only one type of bacteria is present and thus aids us in the study, characterizing and identifying of microorganisms. D. What is aseptic technique? Why is it so critical? Aseptic technique refers to a procedure that is preformed under sterile conditions.Show MoreRelatedA Brief Note On The Integrated Lab Class844 Words   |  4 PagesHarish Singh Pharm 858 Journal Reflection #3 In the first week of the Integrated Lab class focused on becoming familiar with HER during which we learned how to navigate and access important patient information. EHR is utilized by all healthcare professionals to document all therapeutic interventions. Documenting everything efficiently and accurately ultimately leads to better patient care. I believe by being accustomed to using and documenting in an EHR, pharmacy students can be better preparedRead MoreMicroscope Lab Essay701 Words   |  3 Pages Virtual Lab Unit 3 1. Briefly describe the steps required to  aseptically  transfer bacteria from an unknown to a tube of liquid broth.   You might have to research this using the T button (Tell Me More About...) To aseptically transfer bacteria from an unknown to a tube of liquid broth you have to flame the loop or wire before you begin to sterilize it. You then remove the caps from the tubes and flame the mouths of the tubes to prevent air-borne contamination. When all this is done, you haveRead MoreBacteria and Degrees Celsius Essay765 Words   |  4 PagesAseptic Technique Culturing Microbes Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth. Procedure: Obtained a small Styrofoam cooler placed two small light bulbs in side and observed temperature over 24 hours to ensure temperature could be maintained between 98-100 degrees. Using a 10% bleach solution I then cleaned my work area. TransferringRead MoreThe Use Of Biogenesis Theory And Its Effect On Microbial Growth1500 Words   |  6 PagesPasteur that was able to propose a special technique where it allows many scientist to transfer and grow certain microorganisms through culture media. Therefore, the purpose of today s lab is all about transferring and growing microbial growth. In today s lab, the students are going to learn and focus more about the process of subculturing, how to handle an aseptic technique, how it can be beneficial to us, and why is it important to us. And by doing this lab exercise, the students will have to transferRead MoreEssay Aseptic Technique and Bacterial Anatomy and Morphology1896 Words   |  8 PagesLab, Week ASEPTIC TECHNIQUE AND BACTERIAL ANATOMY AND MORPHOLOGY Introduction Part I: Aseptic Technique The purpose of this experiment is to become familiar with the specific microbiological technique known as the aseptic technique, which is used to avoid contaminating cultures. In this case a pure culture of an unknown organism was introduced to a sterile medium of Phenol Red Glucose Broth Durham. The culture was obtained from a 52-year old male truck driver who is complainingRead MoreAseptic Technique And Culturing Bacteria1197 Words   |  5 PagesMATERIAL and METHODS: Aseptic Technique and Culturing Microbes(1) experiment was followed as stated in the lab manual from Clinical Microbiology Class C-453. Aseptic technique was initiated at the beginning of each experiment. Starting by cleansing the work surface with disinfected wipes to prevent cross contamination each time. Utilizing the gloves and personal protective equipment assisted in maintaining a pure culture during the series of experiments. The first step, was to grow the yeastRead MoreBacteria And Its Effects On Humans1374 Words   |  6 Pagestrack of the growth characteristics of the two E.coli types, and eventually, be able to accept or refute the hypothesis stating that E.coli/pAMP is resistant to ampicillin. METHODS The first part of this lab consisted of practicing two basic but important techniques: pipetting and the aseptic technique including the sterile use of a standard pipet. The first pipetting exercise was about getting familiar with using a digital micropipette, with different tip ranges (0-10 ÃŽ ¼L, 0-20 ÃŽ ¼L and 100-1000 ÃŽ ¼L). TheRead MoreGrain Staining1116 Words   |  5 PagesIntroduction The purpose of exercise 4 was to understand the differential staining techniques: gram staining and special stain structures. Differential staining techniques are used to help identify microorganisms based on the morphology and its ability to accept the stains used. During the staining process the cells retaining the initial stain are positive, and the cells retaining the counter or secondary stain are considered to be negative. Differential stains are used to identify microorganismRead MoreThe Organisms And Its Effects On Our Daily Environmental Surfaces And Primary Areas Of Life Essay1711 Words   |  7 Pageson a college campus that are assumed to be dense with bacteria, would the intensity of growth be greater than samples taken from the human body? Material and Methods Students were provided with the following equipment in a sterilized Microbiology Lab. Cotton Swabs †¢ Distilled Water †¢ Test Tubes with caps †¢ Petri Dishes †¢ Nutrient Agar †¢ Blood Agar †¢ Inoculation Loop †¢ Bunsen Burner †¢ Slides †¢ Pipette †¢ Compound Light Microscope †¢ Various Reagents. Students were asked to gather samples from fourRead MoreWhat are Aseptic Techniques?1230 Words   |  5 PagesIntroduction Aseptic techniques were practised by microbiologists in order to eliminate unwanted microorganisms in their working environment, protecting those microbiologists from the potential pathogenic microorganisms and preventing the contaminations of microorganisms to the working environment (Henretig King, 2008). One of the ways for practising of aseptic technique is the sterilization of lab equipment such as glassware; liquids such as diluents can be sterilize using the autoclave machine

No comments:

Post a Comment

Note: Only a member of this blog may post a comment.